Background: In addition to their value as livestock, pigs are susceptible to classical swine fever virus (CSFV) and can\nserve as reservoirs for CSFV, allowing it to develop into an epizootic. CSFV, a pestivirus of the Flaviviridae family, has\na single-stranded RNA genome. Recent research has indicated that the human MxA protein inhibits the life cycles\nof certain RNA viruses, such as members of the Bunyaviridae family, the Flaviviridae family and others.\nResults: To produce pigs with antiviral protection against CSFV, transgenic pigs expressing human MxA were\ngenerated by nuclear transplantation. Cells from three MxA transgenic piglets were used to investigate in vitro\nantiviral activity of MxA aganist CSFV, and the results of in vitro indirect immunofluorescence assays, virus titration\nand real-time PCR indicated that the MxA transgenic pig has an antiviral capacity against CSFV.\nConclusions: Transgene with human MxA on pigs is feasible. High levels of MxA expression do inhibit CSFV in vitro\nat early time points post-infection at 60-96dpi.
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